Research Paper Volume 11, Issue 24 pp 11803—11813

LncRNA HIF1A-AS2 promotes osteosarcoma progression by acting as a sponge of miR-129-5p

Figure 2. HIF1A-AS2 promoted osteosarcoma cell proliferation, cell cycle progression and invasion. (A) The expression of HIF1A-AS2 in osteosarcoma cell lines (U2OS, SoSP-M, SaOS-2, MG-63) and an osteoblast cell line (hFOB) was measured by qRT-PCR. (B) The expression of HIF1A-AS2 in U2OS cells was determined by qRT-PCR. (C) The expression of HIF1A-AS2 in MG-63 cells was determined by qRT-PCR. (D) Ectopic expression of HIF1A-AS2 increased the S phase of U2OS cells. (E) Ectopic expression of HIF1A-AS2 promoted the S phase of MG-63 cells. (F) Overexpression of HIF1A-AS2 promoted U2OS cell proliferation. (G) Ectopic expression of HIF1A-AS2 promoted MG-63 cell growth. (H) Ectopic expression of HIF1A-AS2 increased U2OS cell invasion, and the relative invasive cells are shown. (I) Ectopic expression of HIF1A-AS2 increased MG-63 cell invasion, and the relative invasive cells are shown. *p<0.05, **p<0.01 and ***p<0.001.