Research Paper Volume 11, Issue 24 pp 11829—11843

Suppression of FADS1 induces ROS generation, cell cycle arrest, and apoptosis in melanocytes: implications for vitiligo

Figure 6. Downregulation of FADS1 induces ROS generation, decreases MMP, and promotes expression of apoptosis markers in PIG1 cells. PIG1 melanocytes were transfected with NC-siRNA or FADS1-siRNA2 for 24 h or 72 h. (A, B) ROS generation measured by DCF fluorescence using flow cytometry. (C) Changes in MMP assessed through JC-1 staining and flow cytometry. (D) The ultrastructural morphological changes of mitochondria in PIG1 cells observed on the TEM. (E) Expression of Bax, Bcl-2, active caspase 9, and active caspase 3 detected by western blotting. β-actin was used as internal control. (FI) Relative expression of Bax, Bcl-2, active caspase 9, and active caspase 3 after normalization to β-actin. **P < 0.01, compared with NC cells.