Research Paper Volume 11, Issue 24 pp 11865—11879

MicroRNA 27b promotes cardiac fibrosis by targeting the FBW7/Snail pathway

Figure 4. miR-27b modulated the expression of Snail by inhibiting its degradation (A, B) The mRNA level of Snail in CFs treated with AngII combined with miR-27i (A) or miR2-7b (B). (C) The effect of miR-27b on the expression of Snail in CFs treated with cycloheximide (CHX, 1 μg/ml) at indicated time points. (D) Roles of miR-27b in Snail ubiquitination. (E) Characterization of the messenger RNA (mRNA) of FBW7, which depicted miR-27b binding site (BS) in its 3′-untranslated region (UTR). (F) The interaction of FBW7 and Snail in CFs. (G) The effect of FBW7 overexpression on the expression of Snail in CFs treated with cycloheximide (CHX, 1 μg/ml) at indicated time points. (H) The effect of FBW7 overexpression on the ubiquitination of Snail. Arrow indicates the GFP-FBW7. Data were represented as mean ± SEM (n=4). N, p>0.05.