Research Paper Volume 11, Issue 24 pp 11865—11879

MicroRNA 27b promotes cardiac fibrosis by targeting the FBW7/Snail pathway

Figure 5. FBW7 was the miR-27b target in cardiac fibrosis (AC) The mRNA levels of FBW7 in serum-treated CFs (A), AngII (B), or miR-27b (C). (D) Snail expression in CFs transfection with miR-27b and/or peGFP-FBW7 plasmids. (E, F) The proliferation of CFs treated in (D) were analyzed by MTT test (E), and BrdU assay (F). (G) CFs transfected with miR-27i and/or FBW7 siRNA were subjected to AngII treatment as indicated. Snail expression was analyzed using WB. (H, I) The proliferation of CFs treated in (G) was analyzed by MTT test (H), and BrdU assay (I). Data were represented as mean ± SEM (n=4). *, p<0.05; **, p<0.01.