Figure 3. KIF20A is significantly upregulated in CRC. (A) Immunohistochemical analysis of KIF20A in paraffin tissues from CRC tissues and normal colorectal tissues of two patients (case 1 and case 2). (B) Western blot analysis of KIF20A in five pairs of CRC and paracancerous tissues. (C) mRNA level of KIF20A in twelve pairs of CRC and paracancerous tissues were detected by RT-PCR. Data are presented as mean ± SEM. *P < 0.05. (D–E) Western blot and RT-PCR analysis of KIF20A in a normal colorectal cell line (NCM) and seven CRC cell lines. Data are presented as mean ± SEM.