Research Paper Volume 11, Issue 24 pp 11937—11954

Hsa_circ_0006948 enhances cancer progression and epithelial-mesenchymal transition through the miR-490-3p/HMGA2 axis in esophageal squamous cell carcinoma

Figure 3. The function of hsa_circ_0006948 in ESCC cells. (A) Expression of hsa_circ_0006948 and FNDC3B mRNA in TE-1 cells transfected with siRNAs. and (B) KYSE30 cells overexpressing hsa_circ_0006948. (C and D) The effect of hsa_circ_0006948 on cell proliferation in vitro using colony formation assay and CCK8 assay after knocking down hsa_circ_0006948 in TE-1. (E) Cell migration and invasion abilities were assessed by transwell assay after knocking down hsa_circ_0006948 in TE-1 cells. (F and G) The effect of hsa_circ_0006948 on cell proliferation in vitro using colony formation assay and CCK8 assay after overexpressing hsa_circ_0006948 in KYSE30 cells. (H) Cell migration and invasion abilities were assessed by transwell assay after overexpressing hsa_circ_0006948 in KYSE30 cells. (I) Western blot analysis comparing downregulated and upregulated-hsa_circ_0006948 ESCC cells with control cells were shown for vimentin, E-cadherin and N-cadherin* P<0.05,**P<0.01, ***P<0.001.