Figure 2. LINC00682 knockdown induces miR-9 upregulation but LMX1A downregulation, promoting AGS cell survival, proliferation, migration and invasion. AGS cells were transfected with 500 nM of LINC00682 siRNA (“Seq1/Seq2”, two rounds, total 48h) or the scramble non-sense control siRNA (“si-C”), expression levels of LINC00682 (A), miR-9 (B), LMX1A mRNA (D), LMX1B mRNA (F) were tested by qPCR; The relative LMX1A3’-UTR luciferase activity was tested (C); Expression of the listed proteins in total cell lysates was tested by Western blotting (E); Cells were further cultured for the indicated time periods, cell survival and proliferation in vitro were tested by the appropriate assays (G-I); Cell migration and invasion were tested by “Transwell” (J) and “Matrigel Transwell” (K) assays, respectively. Listed proteins were quantified and normalized to the loading control (E). For each assay, n=5 (five dishes or wells). *P <0.05 vs. “si-C” cells. Experiments in this figure were repeated four times, and similar results were obtained.