Figure 10. Hydrogen-rich medium increased M2 macrophage-associated anti-inflammatory cytokine levels and reduced M1 macrophage-associated pro-inflammatory cytokine levels in RAW 264.7 cells after induction. Secretion of IL-6, TNF-α, IL-10 and TGF-β1 from macrophages. n=8 per group. Data are shown as the mean ± SEM. Significance was calculated by one-way ANOVA, *: P<0.05. Abbreviations: Con, control group; LPS/IFN-γ, RAW 264.7 cells treated with LPS and IFN-γ to induce polarization into M1-type macrophages; HRS, RAW 264.7 cells treated with hydrogen-rich medium only; HRS + LPS/IFN-γ, RAW 264.7 cells treated with hydrogen-rich medium after being treated with LPS and IFN-γ.