Figure 8. Hydrogen-rich medium reduced M1-type macrophage polarization in RAW 264.7 cells after induction. Flow cytometry of F4/80, CD80 double-positive macrophages (i.e., M1-type macrophages). n=4 per group. Data are shown as the mean ± SEM. Significance was calculated by one-way ANOVA, *: P<0.05. Abbreviations: Con, control group; LPS/IFN-γ, RAW 264.7 cells treated with LPS and IFN-γ to induce polarization into M1-type macrophages; HRS, RAW 264.7 cells treated with hydrogen-rich medium only; HRS + LPS/IFN-γ, RAW 264.7 cells treated with hydrogen-rich medium after being treated with LPS and IFN-γ.