Research Paper Volume 11, Issue 24 pp 12114—12130

Reduced miR-203 predicts metastasis and poor survival in esophageal carcinoma

Figure 4. miR-203 inhibits migration and invasion of EC cells through directly targeting KIF5C expression. (A) The targets of miR-203 in EC predicted by TargetScan7.1. (B) Pathway enrichment of 162 potential targets of miR-203. (C) mRNA expression of potential direct targets in tumor tissues and adjacent normal tissues of EC patients was revealed by mRNA-seq provided by TCGA. Data is presented as mean ± SD. ***p < 0.001, **p < 0.01 (vs. adjacent normal tissues). (D) Scatterplot depicts a significant inverse correlation between miR-203 and KIF5C mRNA expression. KIF5C expression in normal esophageal cell line HEEC and EC cell lines KYSE30 and KYSE510 was determined by qRT-PCR (E) and western blotting (F). GAPDH was used as an internal control. Data is presented as mean ± SD from three independent experiments. ***p < 0.001 (HEEC vs. KYSE30), ###p < 0.0001 (KYSE510 vs. KYSE30). Effect of miR-203 inhibitor on KIF5C mRNA (G) and protein (H) expression was determined in EC cells. Effect of miR-203 mimics on KIF5C mRNA (I) and protein (J) expression was determined in EC cells. Data is displayed as the Mean ± SD of three independent experiments. ***p < 0.001, ###p < 0.001. (vs. miR-NC: miR-203 negative control).