Research Paper Volume 11, Issue 23 pp 11565—11575

Nrf2 activation mediates tumor-specific hepatic stellate cells-induced DIgR2 expression in dendritic cells

Figure 4. tHSCs-induced Nrf2 activation in mDCs contributes to inhibition on cytokines. The stable bone marrow-derived dendritic cells (mDCs), with Nrf2 shRNA (“sh-Nrf2”) or Nrf2 KO construct (“ko-Nrf2”), as well as the parental control mDCs (“Pare”), were co-cultured with tumor HSCs (tHSCs) for 72h, mRNA expression (qPCR assay, AC) and protein contents (in conditional medium, ELISA, DF) of listed cytokines were tested. Data are presented as the mean ± standard deviation (n=5). “Ctrl” stands for mDCs only. * P < 0.05 vs. “Ctrl”. # P < 0.05 vs. “Pare” group. The experiments in this figure were repeated four times, and similar results were obtained.