Research Paper Volume 11, Issue 23 pp 11565—11575

Nrf2 activation mediates tumor-specific hepatic stellate cells-induced DIgR2 expression in dendritic cells

Figure 5. Nrf2 activation mediates splenic T cell inhibition by tHSCs-stimulated mDCs. The stable bone marrow-derived dendritic cells (mDCs), with Nrf2 shRNA (“sh-Nrf2”) or Nrf2 KO construct (“ko-Nrf2”) as well as the parental control mDCs (“Pare”) were co-cultured with tumor HSCs (tHSCs) for 24h; Stimulated mDCs were then co-cultured with splenic T cells; OVA-II peptide CTL assay activity (after 24h, A) and LPS (100 ng/mL)-induced IFN-γ production (after 24h, B) were tested. Data are presented as the mean ± standard deviation (n=5). “Ctrl” stands for mDCs only. * P < 0.05 vs. “Ctrl”. # P < 0.05 vs. “Pare” group. The experiments in this figure were repeated three times, and similar results were obtained.