Figure 1. XIAP is down-regulated in fatty liver of mice fed with HFD. (A) qPCR, immunoblotting and (B) immunohistochemical analysis of XIAP expression in liver tissues from scrambled RNA mice fed a SCD or HFD for 12 weeks. (C) Primary mice hepatocytes and normal AML-12 cells were treated with 250 μM palmitate (PA) for 0, 4, 8, 12 or 24 hours, followed by determination of XIAP expression in mRNA and protein levels using qPCR or western blotting detection. For all bar plots shown, data are expressed as the mean ± SEM. n = 8 per group. ap < 0.05 vs. SCD or control group (Ctrl).