Research Paper Volume 11, Issue 24 pp 12328—12344

Overexpressed methyltransferase-like 1 (METTL1) increased chemosensitivity of colon cancer cells to cisplatin by regulating miR-149-3p/S100A4/p53 axis

Figure 3. The involvement of METTL1/miR-149-3p axis in the regulation of CR-CC cell apoptosis. (A, B) FCM was employed to determine the apoptosis ratio of CR-CC cells. (C, D) Western Blot was used to detect the expression levels of cleaved Caspase-3 in CR-HCT116 cells, CR-SW480 cells and CR-SW620 cells respectively. (“Con” represented “Control group”, “Cis” indicated “Cisplatin treated group”, “Cis+OE-MET” represented “Cisplatin plus overexpressed METTL1 treated group”, “Cis+OE-MET+Inhi” represented “Cisplatin plus overexpressed METTL1 and miR-149-3p inhibitor treated group”). All the experiments repeated at least 3 times. “*” means p < 0.05, “**” means p < 0.01 and “NS” means no statistical significance.