Figure 2. CircRNA_100367 exists in KYSE-150R cells with higher potency of endothelial to mesenchymal transformation (EMT). (A) The protein levels characteristic molecules (β-catenin, Vimentin, and Snail) of EMT in KYSE-150 and KYSE-150R cells were detected by western blot. (B) The migration of KYSE-150 and KYSE-150R cells were determined by transwell assay. (C) The CD133 positive cells of KYSE-150 and KYSE-150R cells were measured by flow cytometry. (D) Divergent primers and convergent primers were designed, and PCR product of cricRNA 100367 was detected in 1.5% agarose gel electrophoresis. (E) After the treatment of RNase R, circRNA_100367 and DCAF8 mRNA expressions in KYSE-150 and KYSE-150R cells were detected by qRT-PCR. *p<0.05, p<0.01 vs. KYSE-150 or mock.