Research Paper Volume 11, Issue 24 pp 12412—12427

CircRNA_100367 regulated the radiation sensitivity of esophageal squamous cell carcinomas through miR-217/Wnt3 pathway

Figure 5. Effects of Wnt3 on the proliferation and migration of KYSE-150 and KYSE-150R cells. (A) The potential target genes of miR-217 were predicted by bioinformatics softwares (microT-CDS, miRDB and TargetScan). (B) KYSE-150R cells were transfected with miR-217 mimic (or NC) and then irradiated with 0 and 6 Gy X-ray. The mRNA level of Wnt3 was detected by qRT-PCR. **p<0.01 vs NC. (C) The relationship between Wnt3 expression and survival rate of patients with ESCS. (D) KYSE-150 cells were transfected with Wnt3 overexpression vector and KYSE-150R cells were transfected with Wnt3 silence vector. The invasion of KYSE-150 and KYSE-150R cells were detected by transwell assay, and the protein level of nuclear β-catenin (n β-catenin) was determined by western blot. **p<0.01 vs. KYSE-150; #p<0.05 vs. KYSE-150R. (E) KYSE-150 cells were transfected with Wnt3 overexpression vector and KYSE-150R cells were transfected with Wnt3 silence vector. Then cells were irradiated with 0 and 6 Gy X-ray. The proliferation of cells was evaluated by colony formation assay.