Research Paper Volume 11, Issue 24 pp 12476—12496

lncRNA Rmst acts as an important mediator of BMP9-induced osteogenic differentiation of mesenchymal stem cells (MSCs) by antagonizing Notch-targeting microRNAs

Figure 2. Silencing lncRNA Rmst expression reduces BMP9-induced expression of osteogenic, chondrogenic and adipogenic regulators and bone markers in MSCs. (A) Subconfluent iMADs were infected with Ad-BMP9 or Ad-GFP and AdR-simRmst. At the indicated time points, total RNA was isolated and subjected to TqPCR analysis with primers for mouse Runx2, Sox9, Osx, and Pparγ. Gapdh was used as a reference gene. “*” p<0.05 and “**” p<0.001 when compared with the Ad-GFP control group. Each assay condition was done in triplicate. (B) The cDNA samples prepared in (A) were further subjected to TqPCR analysis with primers for mouse Alp, Opn, Ocn and Col1a1. Gapdh was used as a reference gene. “*” p<0.05 and “**” p<0.001 when compared with the Ad-GFP control group. Each assay condition was done in triplicate.