Research Paper Volume 11, Issue 24 pp 12532—12545

Protease-activated receptor 2 (PAR-2) antagonist AZ3451 as a novel therapeutic agent for osteoarthritis

Figure 3. AZ3451 alleviates the IL-1β-induced autophagy downregulation in chondrocytes. (A, B) Representative western blots and quantification data of Atg5, Atg7, Atg12, Beclin1 and LC3 in chondrocytes after stimulation with 10ng/ml IL-1β under different time course. (C, D) Representative western blots and quantification data of Atg5, Atg7, Atg12, Beclin1 and LC3 in chondrocytes as treated above. (E, F) Fluorescence microscopy analysis of chondrocytes transfected with mRFP-GFP-LC3 adenovirus in each group. Data are shown as the mean ± SD. Significant differences between groups are indicated as ***P <0.001, **P < 0.01 and *P < 0.05.