Research Paper Volume 11, Issue 24 pp 12641—12660

Macrophage migration inhibitory factor rejuvenates aged human mesenchymal stem cells and improves myocardial repair

Figure 2. MIF inhibited the cellular senescence of MSCs. (A) Western blotting and quantitative analysis of MIF protein expression in young and aged MSCs. (B) Western blotting and quantitative analysis of MIF, p53 and p21 protein expression in young MSCs treated with control siRNA or MIF-siRNA. (C) Representative images of SA-β-gal staining and quantitative analysis of SA-β-gal-positive cells in young MSCs treated with control siRNA or MIF-siRNA. (D) Western blotting and quantitative analysis of MIF, p53 and p21 protein expression in aged and MIF-aged MSCs. (E) Representative images of SA-β-gal staining and quantitative analysis of SA-β-gal-positive cells in aged and MIF-aged MSCs. (F) Cell growth curves showed that overexpression of MIF in aged MSCs increased their growth rate. Scale bar=100 μm. Data are expressed as the mean±SEM. n=3. **p<0.01; ***p<0.001.