Research Paper Volume 11, Issue 24 pp 12641—12660

Macrophage migration inhibitory factor rejuvenates aged human mesenchymal stem cells and improves myocardial repair

Figure 3. MIF rejuvenated aged MSCs by promoting autophagy. (A) Western blotting and quantitative analysis of LC3I/II, Beclin1, p62, p53 and p21 protein expression in aged MSCs and MIF-aged MSCs with or without 3-MA treatment. (B) Representative images of TUNEL staining and quantitative analysis of the apoptosis of aged MSCs and MIF-aged MSCs with or without 3-MA treatment under an SD/H challenge. Scale bar=100 μm. Data are expressed as the mean±SEM. n=3. **p<0.01; ***p<0.001.