Research Paper Volume 11, Issue 24 pp 12733—12753

The loss of dopaminergic neurons in DEC1 deficient mice potentially involves the decrease of PI3K/Akt/GSK3β signaling

Figure 5. Levels of DEC1 expression in the midbrain and hippocampus in WT mice. (A) Dual staining with TH (red), DEC1 (green) and DAPI (blue) in the SNpc and hippocampus by immunofluorescence in WT mice (n=6). (B) DEC1 expression in the midbrain and hippocampus was analyzed by Western blotting in WT mice (n=6 in each group) at the age 6 months. The data are analyzed using t-test and expressed as mean ± SD. ***p<0.001, DEC1 expression in the hippocampus vs that in the midbrain. Scale bar=100 μm.