Research Paper Volume 11, Issue 24 pp 12754—12772

Expression profile-based screening for critical genes reveals S100A4, ACKR3 and CDH1 in docetaxel-resistant prostate cancer cells

Figure 6. Cell viability and migration effects after treatment with ACKR3 knockout and/or S100A4 knockdown. Western blots (A) ACKR3 knockout and (B) S100A4 knockdown. (C) Photomicrographs of DU145R and PC3R cells 48 h after ACKR3 knockout and/or S100A4 knockdown. (D and E) Relative viability rates among DU145R and PC3R cells after 24, 48 and 72 h under the indicated treatment condition. Viability among control cells was assigned a value of 100%. (F) Photomicrographs of migration of Doc-resistant DU145R and PC3R cells in wound healing assays 48 h after ACKR3 knockout and/or S100A4 knockdown. (G and H) Relative quantification of migration in wound healing assays under the indicated treatment condition. Migration of control cells was assigned a value of 100%. *P < 0.05, **P <0.01, ***P < 0.001.