Research Paper Volume 11, Issue 24 pp 12773—12792

KIFC1 is essential for normal spermatogenesis and its depletion results in early germ cell apoptosis in the Kuruma shrimp, Penaeus (Marsupenaeus) japonicus

Figure 10. Effects of kifc1 knockdown on P. japonicus testis detected using the TUNEL assay. (A) No apoptosis signal was detected in the normal testes. (B) No apoptosis signal was detected in dsEGFP testes. (C) Apoptosis signal was detected in dsKIFC1-1 testes. (D) No apoptosis signal was detected in dsKIFC1-1 vas deferens. (E) No apoptosis signal was detected in dsKIFC1-1 spermatophore. (F) In dsKIFC1-1 testes, apoptosis was taken place in spermatogonia (white arrows) and spermatocytes (yellow arrows). Blue: DAPI, Red: TUNEL. Scale bar = 20μm in A, B and C. Scale bar = 10μm in D, E and F.