Research Paper Volume 11, Issue 24 pp 12773—12792

KIFC1 is essential for normal spermatogenesis and its depletion results in early germ cell apoptosis in the Kuruma shrimp, Penaeus (Marsupenaeus) japonicus

Figure 8. Knockdown efficiency of kifc1 by dsRNA injection in vivo. (A) Expression level of kifc1 mRNA was assayed by semi-quantitative RT-PCR analysis in the testis, vas deferens and spermatophore of P. japonicus. (B) A quantitative analysis of kifc1 mRNA expression in the testis, vas deferens and spermatophore was determined by Image J. (C) Expression level of KIFC1 protein was detected by Western blot in the testis, vas deferens and spermatophore of P. japonicus. (D) A quantitative analysis of KIFC1 protein expression in the testis, vas deferens and spermatophore was determined by Image J. β-actin was used as an internal control.