Figure 8. Expression of Klotho, Wnt1, Wnt4, Wnt7a in the cell supernatant and GSK3β, p-GSK3β, MMP2, MMP9, MMP-7, β-catenin (cytoplasm/nucleus) in the cell of chondrocytes knockdown of β-catenin. (A) The protein expression detection of Klotho, Wnt1, Wnt4, Wnt7a in the cell supernatant and Phos-GSK3β (S9), GSK3β, MMP2, MMP9, and (B) β-catenin (cytoplasm/nucleus) by Western Bolt, quantitative data (C) is presented. *P,0.05 versus controls (n=3), (E, F) RT-PCR was used to detect the expression of MMP-2 and MMP-9 mRNA in different groups. Results were obtained via the expression of three individual experiments performed in triplicate for each condition. *P < 0.05, **P < 0.01.