Research Paper Volume 12, Issue 2 pp 1141—1158

MiR-29b-3p promotes particulate matter-induced inflammatory responses by regulating the C1QTNF6/AMPK pathway

Figure 4. C1QTNF6 overexpression attenuated PM-induced inflammatory responses. HBECs were transfected with C1QTNF6-overexpressing virus or negative control virus (EV). The C1QTNF6 overexpression efficiency was confirmed using real-time PCR (A) and western blot analysis (B). The optical densities of protein bands were shown in (C). Values represent mean ± SEM; *, P<0.05, compared with the EV group; n=3. (D) Real-time PCR analysis of IL-1β, IL-6, and IL-8 expression in C1QTNF6-overexpressing or negative control HBECs treated with or without 300 μg/cm3 PM for 24 h. (E) Western blot analysis of AMPK signaling pathway activation in C1QTNF6-overexpressed or negative control HBECs treated with or without 300 μg/cm3 PM for 24 h. The optical densities of protein bands were shown in (F). Values represent mean ± SEM; *, P<0.05, compared with the EV + PM group; #, P<0.05, compared with the EV group; n=3. EV, empty vector; HBECs, human bronchial epithelial cells; PM, particulate matter.