Research Paper Volume 12, Issue 2 pp 1213—1236

MicroRNA-200a induces immunosuppression by promoting PTEN-mediated PD-L1 upregulation in osteosarcoma

Figure 3. miR-200a inhibited the function of CD8+ T cells through PD-L1/PD-1 pathway in vitro. (AB) Examine the apoptosis of CD8+ T cells after co-cultured with U2OS miR-200a control or miR-200a OE separately. (CD) Examine the proliferation rate of CD8+ T cells after co-cultured with U2OS miR-200a control or miR-200a OE separately. (EF) Enzyme-Linked ImmunoSorbent Assay (ELISA) of the secretion of granzyme B and perforin. *P<0.05, **P<0.01, ***P<0.001.