Research Paper Volume 12, Issue 2 pp 1213—1236

MicroRNA-200a induces immunosuppression by promoting PTEN-mediated PD-L1 upregulation in osteosarcoma

Figure 4. miR-200a promoted tumor growth by up-regulating the expression of PD-L1 in vivo. (A) miR-200a promoted the growth of osteosarcoma through up-regulating PD-L1. (B) Quantification of tumor volume. (CD) Immunohistochemical staining analysis of ki-67 expression in tumor tissues. Scale bar represents 50μm. (E) Immunohistochemical staining analysis of PD-L1 expression in tumor tissues. Scale bar represents 100μm. (FG) Flow cytometry analysis of PD-L1 expression in tumor tissues. *P<0.05, **P<0.01, ***P<0.001.