Research Paper Volume 12, Issue 2 pp 1304—1321

AURKB promotes gastric cancer progression via activation of CCND1 expression

Figure 3. Restoration of CCND1 abrogated the inhibition of cell growth mediated by AURKB-siRNAs. (A) Western blot showing the restoration of CCND1 expression in SGC7901 and BGC823 cells transfected with AURKB siRNA or negative control siRNA. HSP70 was the loading control. (B) Enforced expression of CCND1 significantly abrogated the inhibition of proliferation mediated by AURKB siRNA in SGC7901 and BGC823 cells, as evidenced by CCK-8 assays. The results shown are the means ± SDs of three independent experiments; **, P < 0.01 compared with the AURKB knockdown groups. (C) Enforced expression of CCND1 significantly abrogated the inhibition of colony formation mediated by AURKB siRNA in SGC7901 and BGC823 cells. Left panel, representative images from colony formation assays. Right panel, the number of colonies formed by the indicated cells was quantified. Data are presented as the means ± SDs; **, P<0.01 compared with the AURKB knockdown groups. (D) Enforced expression of CCND1 significantly abrogated the cell cycle arrest in G2/M phase mediated by AURKB siRNA in SGC7901 and BGC823 cells. Right panel, bar graphs showing the percentages of SGC7901 and BGC823 cells in the G0/G1, S and G2/M phases. Each histogram bar represents the mean ± SD of three independent experiments.