Research Paper Volume 12, Issue 2 pp 1304—1321

AURKB promotes gastric cancer progression via activation of CCND1 expression

Figure 4. AURKB promotes gastric cancer cell growth by regulating CCND1 in vivo. (A), Western blot analysis of the protein levels of AURKB, CCND1 and HSP70 in stable BGC823 cell lines. HSP70 was used as the endogenous loading control. (B) Photograph of xenograft tumors excised from mice in the scrambled (SCR) control, AURKB KD and AURKB KD + CCND1 OE groups. (C) Tumor masses of xenograft tumors excised from mice in the SCR control, AURKB KD and AURKB KD + CCND1 OE groups. Data are presented as the means ± SDs; **, P < 0.01. (D) Tumor volumes were examined on the indicated days. Data are presented as the means ± SDs; *, P < 0.05, **, P < 0.01. (E) Western blotting analysis of AURKB and CCND1 expression in xenograft tumors excised from mice in the SCR control, AURKB KD and AURKB KD + CCND1 OE groups. HSP70 was used as the endogenous loading control. (F) Hematoxylin and eosin (H&E) staining and IHC staining of AURKB, CCND1 and Ki-67 in xenograft tumors excised from mice in the SCR control, AURKB KD and AURKB KD + CCND1 OE groups.