Research Paper Volume 12, Issue 2 pp 1397—1416

Activated Drp1-mediated mitochondrial ROS influence the gut microbiome and intestinal barrier after hemorrhagic shock

Figure 3. The effects of Drp1 on intestinal epithelial tight junction after hypoxia. (A) Drp1 protein expression in hypoxia-treated IECs after Drp1 shRNA (n=5). (B) Mitochondrial morphology in hypoxia-treated IECs after Drp1 shRNA. (Bar, 25μm). The mitochondria skeletons are analyzed by Image J software. (C) The length of mitochondria skeletons of IECs in each group. 69 mitochondria skeletons are observed and measured by Image J software in Normal group, 139 mitochondria in Hypoxia group, 118 mitochondria in Hypoxia+ Vector group and 35 mitochondria in Hypoxia+Drp1 shRNA group. (D) Tight junctions (ZO1) of IECs in each group detected by immunofluorescence. (Bar, 50μm). (E) The TEER value of monolayer IECs in each group. The observation time lasts 12 hours and measures every 1 hour (n=5). (F) The Zonulin content in supernatant of IECs in each group (n=8). a represents p < 0.05 compared with Normal group; b represents p < 0.05 compared with Hypoxia group.