Research Paper Volume 12, Issue 2 pp 1417—1432

SRV2 promotes mitochondrial fission and Mst1-Drp1 signaling in LPS-induced septic cardiomyopathy

Figure 5. SRV2-induced mitochondrial fission promotes mitochondrial damage. (AB) An ROS probe was used to detect ROS production in cardiomyocytes after transfection of siRNA against SRV2 and addition of FCCP to the culture medium. (CE) SOD, GSH, and GPX levels were measured via ELISA in cardiomyocytes after SRV2 siRNA transfection and FCCP treatment. (F) mPTP opening was determined via ELISA in cardiomyocytes after SRV2 siRNA transfection and FCCP treatment. (GJ) After treatment, RNA was isolated from LPS-treated cardiomyocytes and qPCR was performed to analyze Bcl2, Bad, Bax, and c-IAP transcript levels after SRV2 siRNA transfection and FCCP treatment. *p<0.05 vs. control group, #p<0.05 vs. LPS group, @p<0.05 vs. LPS+si-SRV2 group.