Figure 3. Down-regulation of AQP9 in HCC cells. (A) The mRNA levels of AQP9 in HCC cell lines were determined compared with the control. (B–D) The protein levels of AQP9 in Huh-7 and SMMC-7721 cells transfected with LV-NC or LV-AQP9 were also examined using western blotting. The results were represented as mean ± SD. P<0.05 vs. LO2 or LV-NC. Each experiment was repeated 3 times. AQP, aquaporin 9; HCC, hepatocellular carcinoma; NC, negative control.