Figure 4. Overexpression of AQP9 inhibited proliferation of HCC cells. (A and B) The proliferative activity of Huh-7 and SMMC-7721 cells transfected with LV-AQP9 was determined by CCK-8 assay compared with the control. (C) The expression levels of PCNA in HCC cells were examined following the transfection with LV-NC or LV-AQP9. (D–F) The expression of PCNA in Huh-7 and SMMC-7721 cells transfected with LV-NC or LV-AQP9 were evaluated using immunocytochemistry analysis. The results were represented as mean ± SD. P<0.05 vs. LV-NC. Each experiment was repeated 3 times. AQP, aquaporin 9; HCC, hepatocellular carcinoma; NC, negative control.