Figure 3. UBE2T is overexpressed in HCC tissues and cell lines. (A) SENP1-related interaction network was constructed based on String database. (B) Correlation analysis of SENP1 and UBE2T expression was performed based on gene expression profiling data of TCGA. (C) The protein levels of UBE2T in adjacent normal tissues and tumor tissues were examined by western blot. (D) The average relative protein levels of UBE2T in adjacent normal tissues and tumor tissues obtained from gray analysis of Western blot results. (E) The expression of UBE2T in adjacent normal tissues and tumor tissues was determined by immunohistochemical staining. (F, G) UBE2T expression in TCGA RNAseq database. (H, I) The association between UBE2T expression or SENP1+UBE2T expression and survival rate in TCGA database or KM plotter dataset. (J) Expression of SENP1 and UBE2T were positively related in HCC clinical samples. The data were analyzed with Pearson correlation by GraphPad prism 6.0. The representative images were selected from at least three independent experiments.