Figure 4. Effects of CHS on the TCF7L2 expression levels. (A) βTC3 cell was incubated with CHS (40μM) for series of times (0, 6, 12, 24, 36, 48h), then the TCF7L2 protein expression levels was measured by western blotting. (B) βTC3 cell was incubated with CHS (0, 2.5, 5, 10, 20, 40μM) for 24h, then the TCF7L2 protein expression levels was measured by western blotting. βTC3 cell was incubated with different doses of CHS (C) or different times (D), the mRNA levels of TCF7L2 was measured by RT-PCR. E. Effects of CHS on the protein expression levels of TCF7L2, GLP-1R, GIPR and P53 after IHG or SHG treatment. Data are representative of three independent experiments. ##P<0.01 vs. NG treatment group, **P<0.01 vs. SHG treatment group, ΔΔP<0.01 vs. IHG treatment group.