Research Paper Volume 12, Issue 2 pp 1643—1655

Hsa_circ_0070963 inhibits liver fibrosis via regulation of miR-223-3p and LEMD3

Figure 1. Downregulation of hsa_circ_0070963 in liver fibrosis. (A) Liver fibrosis was confirmed by Masson staining in CCl4-treated mice. The scale bar represents 100 μm. (B) Hsa_circ_0070963 expression was analyzed in primary HSCs isolated from NC or CCl4-treated mice. (C) Relative hsa_circ_0070963 gene expression was detected in the TGF-β1-treated LX2 cell line during culture days. (D) Hsa_circ_0070963 expression was examined in the LX2 cell line treated with increasing concentrations of TGF-β1. (E) Relative hsa_circ_0070963 gene expression was detected in the Sal B-treated LX2 cell line during culture days. (F) Hsa_circ_0070963 expression was examined in the LX2 cell line treated with increasing concentrations of Sal B. Data are presented as means ± SD of three experiments (*p < 0.05 and **p < 0.01).