Research Paper Volume 12, Issue 2 pp 1643—1655

Hsa_circ_0070963 inhibits liver fibrosis via regulation of miR-223-3p and LEMD3

Figure 5. LEMD3 is the target gene of miR-223-3p. LX2 cells were transduced with a miR-223-3p mimic or inhibitor for 48 h. (A) The luciferase activity of 5 predicted mRNA targets in miR-223-3p mimic cells were examined by luciferase assay. (B, C) The mRNA expression of LEMD3 in the LX2 cell line was detected by qRT-PCR. (D, E) The protein expression of LEMD3 in the LX2 cell line was detected by western blot. (F) Predicted binding sites of miR-223-3p in the 3′-UTR of LEMD3. The mutated LEMD3 3′-UTR sequence is presented. (G) Relative luciferase activity was determined at 48 h after transfection with a miR-223-3p NC/mimic/inhibitor or LEMD3-Wt/Mu in HEK293T cells. Data are presented as means ± SD of three experiments (*p < 0.05 and **p < 0.01).