Research Paper Volume 12, Issue 2 pp 1643—1655

Hsa_circ_0070963 inhibits liver fibrosis via regulation of miR-223-3p and LEMD3

Figure 6. Hsa_circ_0070963 modulates the expression of the endogenous miR-223-3p target LEMD3. (AC) LX2 cells were transduced with LEMD3 for 48 h. LEMD3 expression was detected by qRT-PCR (A) and cell proliferation was monitored using the CCK-8 assay (B). Then, the protein levels of α-SMA and Col1A1 were measured by western blot (C). (D, E) LX2 cells were transfected with hsa_circ_0070963 and the mRNA and protein expression levels of LEMD3 were determined by qRT-PCR (E) and western blot (E), respectively. (F) The results of the qRT-PCR showed that miR-223-3p could partly decrease the protein expression level of LEMD3 which were promoted by hsa_circ_0070963. (G) The western blot analysis showed that miR-223-3p could partly decrease the protein expression level of LEMD3 which were promoted by hsa_circ_0070963. Data are presented as means ± SD of three experiments (*p < 0.05, **p < 0.01, and ***p < 0.001).