Research Paper Volume 12, Issue 2 pp 1778—1791

LncRNA DANCR attenuates brain microvascular endothelial cell damage induced by oxygen-glucose deprivation through regulating of miR-33a-5p/XBP1s

Figure 11. XBP1s overexpression reversed the effect of miR-33a-5p on the proliferation and angiogenesis in OGD-treated BMECs. (A) Proliferation was measured by MTS after co-transfection with miR-33a-5p mimic and XBP1s-pcDNA3.1 at 48 h, followed by OGD treatment for 4 h. (BD) The bar represents the apoptotic rate (B), migratory cells (C), and number of meshes (D). (E) A representative image of apoptosis, migration (scale bar: 200×), and angiogenesis (scale bar: 200×), measured by flow cytometry, trans-well, and tube formation assay, respectively, after co-transfection with miR-33a-5p mimic and XBP1s-pcDNA3.1 at 48 h, followed by OGD treatment for 4 h. ***P<0.001 vs ov-NC.