Research Paper Volume 12, Issue 2 pp 1778—1791

LncRNA DANCR attenuates brain microvascular endothelial cell damage induced by oxygen-glucose deprivation through regulating of miR-33a-5p/XBP1s

Figure 7. miR-33a-5p directly bound to DNACR and XBP1. (A) miRNAs bound to DNACR and XBP1 were analyzed using StarBase V2.0 and Targetscan V7.2. (B) miR-33a-5p expression was measured using qRT-PCR after OGD treatment for 0, 2, 4, 6, 8, and 10 h. (C) The relationship between miR-33a-5p expression and DANCR or XBP1s expression was analyzed using Pearson’s analysis. (D) miR-33a-5p expression was measured using qRT-PCR after transfection with DANCR-pcDNA3.1 at 48 h, followed by OGD treatment for 4 h. (E) Predicted binding sites between XBP1 and miR-33a-5p; luciferase reporter assay was conducted to detect luciferase activity after co-transfection of BMECs with WT-XBP1 or MUT-XBP1 and NC, miR-33a-5p mimics. (***P< 0.001). (F) Predicted binding sites between DANCR and miR-33a-5p; luciferase reporter assay was conducted to detect luciferase activity after co-transfection of BMECs with WT-DANCR or MUT-DANCR and NC, miR-33a-5p mimics. (***P< 0.001).