Research Paper Volume 12, Issue 2 pp 1867—1887

miR-106b-5p contributes to the lung metastasis of breast cancer via targeting CNN1 and regulating Rho/ROCK1 pathway

Figure 3. The CNN1 expression was decreased in BRCA tissues and cells. (A) The downregulation of CNN1 in breast cancer tissues (n=20) compared with normal breast tissues (n=20). (B) The protein expression of CNN1 was decreased in BRCA cell lines compared with the healthy breast cell line. *P<0.05 vs. MCF-10A and **P<0.001 vs. MCF-10A. (C) The CNN1 expression was upregulated after CNN1 overexpression transfected MCF-7 and MDA-MB-231 cells. Control, the cells were cultured without any treatment. NC, the cells were treated with the negative control. CNN1 OE, the cells were treated with CNN1 overexpression. *P<0.05 vs. Control and **P<0.001 vs. Control. (D) The CNN1 expression was downregulated after CNN1 small interfering RNA (siRNA) transfected T47D and CAMA-1 cells. Control, the cells were cultured without any treatment. NC, the cells were treated with the negative control. si-CNN1, the cells were transfected with CNN1 siRNA. *P<0.05 vs. Control and **P<0.001 vs. Control.