Research Paper Volume 12, Issue 2 pp 1867—1887

miR-106b-5p contributes to the lung metastasis of breast cancer via targeting CNN1 and regulating Rho/ROCK1 pathway

Figure 4. CNN1 inhibited cell proliferation and migration of breast cancer. (A) and (B) CNN1 overexpression inhibited cell proliferation in MCF-7 and MDA-MB-231 cells, while CNN1 knockdown promoted cell proliferation in T47D and CAMA-1 cells. CCK8 was performed to detect the ability of cell proliferation after the breast cancer cells (MCF-7, MDA-MB-231, T47D, and CAMA-1 cells) treated with negative control, CNN1 overexpression or CNN1 siRNA for 0 h, 24 h, 48 h, and 72 h. (C) and (D) CNN1 overexpression inhibited cell invasion in MCF-7 and MDA-MB-231 cells, while CNN1 knockdown promoted cell invasion in T47D and CAMA-1 cells. The cell invasion after CNN1 overexpression or CNN1 knockdown for 24 h was measured using transwell invasion assay. Control, the cells were cultured without any treatment. NC, the cells were treated with the negative control. CNN1 OE, the cells were treated with CNN1 overexpression. si-CNN1, the cells were transfected with CNN1 siRNA. *P<0.05 vs. Control and **P<0.001 vs. Control.