Research Paper Volume 12, Issue 2 pp 1867—1887

miR-106b-5p contributes to the lung metastasis of breast cancer via targeting CNN1 and regulating Rho/ROCK1 pathway

Figure 5. CNN1 overexpression suppressed cell migration, colony formation, and cell adhesion, while CNN1 overexpression enhanced the abilities of cell apoptosis. (A) The role of CNN1 overexpression in cell migration was confirmed using wound healing assay. The MCF-7 and MDA-MB-231 cells were treated with CNN1 overexpression for 0 h, 24 h, and 48 h. (B) The ability of colony formation was confirmed using colony formation assay. The MCF-7 and MDA-MB-231 cells were treated with negative control or CNN1 overexpression for 14 days. (C) The ability of cell adhesion after CNN1 overexpression for 30 min and 60 min was assessed using the cell adhesion assay. (D) The flow cytometry was performed to measure the cell apoptosis rate after CNN1 overexpression. Control, the cells were cultured without any treatment. NC, the cells were treated with the negative control. CNN1 OE, the cells were treated with CNN1 overexpression. *P<0.05 vs. Control and **P<0.001 vs. Control.