Figure 5. BMP4 down-regulates the mTORC1 signaling pathway in mouse liver. The liver samples prepared in Figure 3 were used for the following assays. (A) Total cell lysate was prepared from the retrieved liver samples and subjected to Western blotting analysis of the expression of the members of mTORC1 signaling pathway. (B) The retrieved liver samples were paraffin-embedded, sectioned and subjected to IHC staining to detect the expression of the members of mTORC1 signaling pathway and lipid metabolism. Each assay condition was done in triplicate, and representative images are shown.