Figure 6. SERPINH1 expression regulates the proliferation and survival of GC cell lines. (A) Western blot analysis shows that SERPINH1 protein levels are higher in the four GC cell lines, HGC-27, AGS, MGC-803, SGC-7901, compared to the normal gastric mucosal cell line, GES-1. (B) Western blot analysis shows that SERPINH1 protein levels are significantly reduced in shSERPINH1 #1-transfected SGC-7901 cells compared with SGC-7901 cells transfected with shSERPINH1 #2, shSERPINH1 #2, and shNC. (C) Representative images of colonies in shSERPINH1#1-transfected SGC-7901 cells, SERPINH1-overexpression vector transfected MGC-803 cells, and their corresponding controls. (D) Histogram plots show the number of colonies in shSERPINH1#1-transfected SGC-7901 cells, SERPINH1-overexpression vector-transfected MGC-803 cells, and their corresponding controls. (E) Flow cytometry analysis shows that apoptotic rate is significantly higher in the shSERPINH1#1-transfected SGC-7901 cells and significantly lower in the SERPINH1-overexpression vector-transfected MGC-803 cells compared to their corresponding controls. (F) Histogram plot shows the percentage of apoptotic cells in shNC-, and shSERPINH1#1-transfected SGC-7901 cell cultures, as well as, empty vector and SERPINH1-overexpression vector-transfected MGC-803 cells.