Research Paper Volume 12, Issue 4 pp 3574—3593

SERPINH1 regulates EMT and gastric cancer metastasis via the Wnt/β-catenin signaling pathway

Figure 9. Anti-tumor effects of CO1003 in GC cells. (A) CCK-8 assay analysis shows concentration-dependent inhibition of proliferation of SGC-7901 GC cells by CO1003 (0, 0.01, 0.1, 1, 10, 100, 1000 μM). This curve was used to determine the IC50 concentration of CO1003, which is the concentration of CO1003 required to reduce proliferation of SGC-7901 cells by 50%. IC50 for CO1003 is 47.56 μM. (B) Transwell migration and invasion assay shows reduced migration and invasion of CO1003-treated SGC-7901 cells compared to the DMSO-treated GC cells. (C, D) Histogram plots show total number of (C) migrating and (D) invading DMSO- and CO1003-treated SGC-7901 cells. (E) Representative images of wound healing assay show that the distance between wound edges was significantly higher in Co1003-treated SGC-7901 cells compared with the DMS-treated SGC-7901 cells. (F) Wound healing assay shows lower wound healing rate because of reduced migration in CO1003-treated SGC-7901 cells compared with the DMSO-treated SGC-7901 cells.