Figure 3. GUSBP5-AS have a central role in proliferation. (A) Cell proliferation ability of GUSBP5-AS in lncRNA GUSBP5-AS knockdown and overexpression group and their corresponding NC groups was detected by CCK8 assay. *P< .05, **P< .01 compared to the LV3-NC group; #P < .05, ##P < .01 compared to the LV5-NC group. (B) The effect of GUSBP5-AS on EPC cycle was investigated by FCM cell cycle distribution test. (C) The effect of lncRNA GUSBP5-AS on cell apoptosis was measured with Annexin V-APC/7-AAD Apoptosis Detection Kit by FCM. Cell apoptosis was analysed. Data from 3 experiments are summarized. *P< .05, **P< .01 compared to the corresponding negative control group. Data are represented as mean ± SEM.