Figure 8. GUSBP5-AS regulates FGF2 and MMP2/9 expression through FOXO1/Akt pathway by sponging with miR-223-3p. (A) Effects of GUSBP5-AS on the regulation of the protein expression of FOXO1, p-Akt, MMP2, MMP9 and FGF2 in EPCs by western blot analysis. GUSBP5-AS knockdown and overexpression, respectively, reduced and increased these protein expression levels. (B) Western blot analysis of the protein expression levels of FOXO1, p-Akt, MMP2, MMP9 and FGF2 in EPCs after infected with NC, miR-223-3p inhibitor and mimics. The resulted showed that miR-223-3p inhibitor and mimics, respectively, promoted and inhibited these protein expression. *P < .05, **P < .01, ***P < .001 compared to the corresponding control group. Data are represented as mean ± SEM. (C) Schematic of the role of GUSBP5-AS-mediated effects in EPCs. Red arrow indicates inhibition and blue arrow indicates promotion.