Research Paper Volume 12, Issue 7 pp 5625—5639

LncRNA 2310043L19Rik inhibits differentiation and promotes proliferation of myoblast by sponging miR-125a-5p

Figure 3. LncRNA 2310043L19Rik knockdown promotes myoblast differentiation. (A) Real-time PCR analysis of 2310043L19Rik knockdown in C2C12 transfected with control NC or three silnc-231s. (B) Representative photographs of MyHC immunofluorescence staining in C2C12 cells differentiated for 4 d transfected with control NC or silnc-231 showing that lnc-231 promoted myoblast differentiation. Positively stained cells were quantified (C). (D) Real-time PCR analysis of MyHC expression in C2C12 transfected with control NC or silnc-231 then cultured in DM for 4 days. Western blot results showing that 2310043L19Rik knockdown significantly increased the protein expression levels of MyHC (E) and MyoG (F) in C2C12 cells differentiated for 3 d, and relative protein levels were measured.